Kev tshawb fawb tshiab los ntawm Shanghai lub koom haum ntawm MiliCiS of Sphingolip synthesis, uas tau kov yeej cov tshuaj lom neeg, tau kov yeej cov kev ua yeeb yam nyuaj ntawm sphingxtrin (M CD) plays lub luag haujlwm tseem ceeb.
Kev Tshawb Fawb Pawg ua haujlwm tau coj los ntawm lub koom haum Shanghai, tau tsim cov kev npaj ua txuj ci materia, tau tsim kho cov txheej txheem biomimication los ntawm "kev ua tiav kev npaj ua tiav ntawm cov qauv sib txawv thiab cov kab mob sphingol.
Qhov kev kawm no siv tus kheej -} Cycloylic kab ke rau hauv hydrophilic saum toj no, yog li kev txhim kho cov kev vam meej ntawm enzymatic catalytic.
1. Daws cov teeb meem hluavtaws
Sphingolipids yog cov birophilic biomoleculic biomolecules faib rau hauv ntau yam kev paub ntawm tes, kev tiv thaiv kab mob ntawm kev tawm tsam cell, kev khiav tawm thiab cov qog metastas.
Vim yog lawv txoj kev sib xyaw ua ke hetered, nws yog qhov nyuaj heev rau kev ncaj qha cais tawm thiab tau txais zoo}}}}}}}}}}} Nyeem los ntawm cov qauv ntuj. Txawm li cas los xij, cov tshuaj lom neeg hluavtaws hluavtaws tsuas yog txwv los ntawm cov nyiaj npib tsawg ntawm cov kab ke ntawm cov piam thaj thiab lipids.
Cov dej so dej nyab ntawm glycolipid substrates kuj tseem ceeb heev kev kawm ntawm enzymatic cov tshuaj tiv thaiv. Cov xwm txheej ua ke ua rau muaj kev nyuaj loj hauv synthesis ntawm cov sphingolipids muaj ntau hom qauv.
2. Tub ntxhais ntawm kev tsim kho tshiab
Pawg neeg tshawb fawb tau coj los ntawm tus lis khi li lis, luam tawm cov qauv siv hluav taws xob Asmeskas ntawm metyl {0} {cyclodextrin.
Methyl - - cyclodextrin tus kheej {}}}}}}}}}}}}}}}}}} Cov kab ke hydrots}}}
This assembly method provides an ideal interface for enzyme-catalyzed reactions, and the formed glycolipid products can be rapidly purified through C18 solid-phase extraction.
3. Tsim cov piam thaj txawv thiab lipid
Los ntawm kev qhia ua tau zoo thib ob rog dhau los ntawm pab pawg ua haujlwm tau tsim muaj 20 nruab nrab thiab acidic sphingolipids nrog ntau qhov sib txawv thiab sucidification hloov kho.
Lub tsev qiv ntawv Glycolipid no ua rau lub glifass {{0} {{} {2} {{1} {{1} {{2} {{1} {{1} {{1} {{1} {{1} {{1} {{1} {
4. Nws muaj dav thov cov kev cia siab
Ntxiv rau nws daim ntawv thov hauv glycolip synthesis, methylbeta - cyclodextrin nws tus kheej kuj tau pom cov txiaj ntsig dav dav hauv kev kho mob. Nws tuaj yeem txhim kho cov solubility thiab bioavailability ntawm cov tshuaj, nce ntau ntawm cov tshuaj, kho kom muaj tshuaj lom thiab phiv tshuaj, thiab nce cov tshuaj lom neeg.
Tsis ntev los no kev ua yeeb yam qhia tau tias M CD thiab nws cov kev npaj kuj tseem siv tau los npaj cov tswv yim osteogenic inhibitions cov kab mob thiab pob txha hyacture kho cov cai.
Cov tswv yim hluavtaws biomimetic raws li methyl - - cyclodextrin tsis yog ua rau muaj kev sib tw tshiab ntawm cov khoom siv hluav taws xob nyuaj Glycolipids.
